Collagen is an inert, rigid protein found predominantly in skin, ligaments, bones and teeth. Its most distinctive attribute, essential to a transmitter of mechanical force, is inelasticity. Its fundamental structural unit is tropo-collagen, a molecular rod about 2600� in length and 15� in diameter and 300,000 molecular weight. In tendons these macromolecules, grouped as collagen fibrils, run parallel to the axis. In skin the fibrils are interlaced and branched.
Reacts with anti Collagen Type II. Reaction with anti-Collagen I, III, IV, V, or VI is negligible (typically <1% cross reactivity was detected in ELISA).
Source:
Bovine nasal cartilage
Applications:
Suitable for use a control or standard in indirect trapping ELISA for quantitation of antigen in serum using a standard curve, Immunoprecipitation, and Western Blot . Other applications not tested.
Recommended Dilution:
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.