Clostridium difficile Toxoid A is a highly purified, non-toxic preparation derived from C. difficile toxin A. It has been developed either for use as an immunogen to raise antibodies or for use in clinical diagnostic assays. Formaldehyde is use to convert the toxin into Toxoid A and quality control data illustrates that this treatment has no significant effect on the antigenicity of the resultant preparation.
Source:
Native toxin A, strain VPI10463 from C. difficile, converted to inactive toxoid form
Inactivation:
Inactivated by formaldehyde. No cell rounding in the sensitive verocytotoxicity assay at 2ug/ml compared to active toxin lethal dose of 0.2ng/ml.
Antigenicity:
Maintained to be (same/more than)90% of original toxin strength aas measured by Direct ELISA.
Applications:
Suitable for use in Direct ELISA. Other applications not tested.
Recommended Dilutions:
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Note:
No test guarantees a product to be non-infectious. All materials should be handled as if potentially infectious. Generally accepted laboratory practices appropriate for infectious materials should be employed when handling this product.