Chromomycin A3 is the major component of the chromomycin complex of the aureolic acid class, isolated from several Streptomyces species and first reported in 1960. Chromomycin A3 exhibits a broad biological profile as an antibacterial, antifungal and antitumor agent. It binds reversibly to GC-specific DNA ligand in the minor groove which inhibits transcription, DNA gyrase and topoisomerase II. The intense UV spectrum and strong fluorescence makes chromomycin a useful stain for DNA.
Chromomycin A3 from Stretomyces griseus.
Soluble in ethanol, methanol, DMF or DMSO. Limited water solubility.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with DMSO. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.