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Chromatin Immunoprecipitation (ChIP) Assay Kit, BioAssay(TM)

Chromatin Immunoprecipitation (ChIP) Assay Kit, BioAssay(TM)

Cat no: C5069-95

Supplier: United States Biological
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For immunoprecipitation of transcriptionally active chromatin from mammalian cells. Detection of gene or promoter of interest interest in immunoprecipitated chromatin must be empirically determined by the researcher. Quantitative PCR or Southern slot-blot analysis, using promotor-specific primer or probe, is recommended Quantity: 22 Chromatin Immunoprecipitation (ChIP) assays. Kit Components: C5069-95A: Salmon Sperm DNA/Protein A Agarose, 1x1.5ml C5069-95B: ChIP Dilution Buffer, 2x24ml C5069-95C: Low Salt Immune Complex Wash Buffer, 1x24ml C5069-95D: High Salt Immune Complex Wash Buffer, 1x24ml C5069-95E: LiCl Immune Complex Wash Buffer, 1x24ml C5069-95F: TE Buffer, 2x24ml C5069-95G: EDTA, 1x250ul C5069-95H: NaCl, 1x500ul C5069-95J: Tris-HCl, 1x500ul C5069-95K: SDS Lysis Buffer, 1x10ml Storage and Stability: May be stored at 4 degrees C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Catalogue number: C5069-95
Applications: Immunoprecipitation
Size: 1Kit
References: General References: 1. Luo, R.X., et al., Cell 92: 463-473 (1998). 2. Braunstein, M., et al., Mol. Cell. Biol. 16: 4349-4356 (1996). Application References: 3. Manabe, I., et al., J. Clin. Invest. 107: 823-834 (2001). 4. Cervoni, N. & Szyf, M., J. Biol. Chem. 276: 40,778-40,787 (2001). 5. Gummow, B.M., et al., J. Biol. Chem. 278: 26,572-26,579 (2003). 6. Detich, N., et al., J. Biol. Chem. 278: 27,586-27,592 (2003). 7. Sundaraj, K.P., et al., J. Biol. Chem. 279: 6152-6162 (2004).

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