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Cellular Phosphotyrosine ELISA Kit, BioAssay(TM)

Cat no: C2609-50

Supplier: United States Biological
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The cellular phosphotyrosine direct enzyme-linked immunoabsorbant assay (ELISA) kit can be used to characterize tyrosine phosphorylation in adherent cultured cells. It has been shown to be useful for analyzing the effect of inhibitors of cellular tyrosine phosphorylation. This kit requires a model cell line provided by the researcher that can be stimulated in vitro to undergo tyrosine phosphorylation. Human A431 carcinoma cell and foreskin fibroblast cells stimulated with epidermal growth factor as well as mouse 3T3 fibroblasts stimulated with insulin have been successfully used with this kit. Applications: Characterization of tyrosine phosphorylation in adherent cultured cells Capacity: 192 Assays Components: C2609-50A: Phosphotyrosine (HRP) 1x200ug C2609-50B: Blocking Reagent 1x2g C2609-50C: TMB tablets 1x4tablets C2609-50D: Phosphate-Citrate Buffer (5x) 1x8ml C2609-50E: DMSO 1x4ml C2609-50F: Tissue culture plates 2x96wells Other components required but not included as part of kit are: Fresh Hydrogen Peroxide 30% Model cell line Culture media Growth Factor or cytokine Phosphate Buffered Saline (PBS), pH 7.4 Protein tyrosine kinase inhibitors ELISA Plate Reader with filter for single wavelength for 375nm, 450nm, and/or 655nm 37 degrees C incubator timer variable volume (5-200l) pipet + tips 5, 10 and 25ml pipets Acetic acid Methanol 0.5M H2SO4 Storage and Stability: Stable for 6 months at 4 degrees C from date of shipment.
Catalogue number: C2609-50
Applications: ELISA
Size: 1Kit
References: Cohen, B., et al., Proc. Natl. Acad. Sci. USA 87: 4458-4462, 1990. Druker, B.J., et al., New Eng. J. Med. 321: 1383-1391, 1989. Kanakura, Y., et al., J. Biol. Chem. 266: 490-495, 1991.

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