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CD70 (Tnfsf7, CD27 Ligand) (FITC)

Cat no: C2425-01N

Supplier: United States Biological
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Human CD70 is not present on resting lymphocytes. CD70 is expressed after activation on about 20% of T cells and on about 70% of B cells. CD70 is a ligand for CD27 and it is also a member of the tumor necrosis factor gene family. Applications: Suitable for use in ELISA, Flow Cytometry, Immunocytochemistry and Immunohistochemistry. Iinhibits T cell proliferation induced by dendritic cells. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Flow Cytometry Protocol: 1. Five x 10e5 cultured human Raji cells were washed and incubated 45 minutes on ice with 80 ml of C2425-01N at a 1:50 dilution factor (10ug/ml). 2. Cells were washed three times, fixed and analyzed on a BD FACstar plus. 3. Cells stained positive with a mean shift of 0.8 log10 fluorescent units when compared to a Mouse IgG1/FITC negative control at a similar concentration. Binding was blocked when cells were pre incubated 10 minutes with 20ul of 0.5mg/ml C2425-01L CD70 antibody.
Catalogue number: C2425-01N
Reactivities: Human
Hosts: Mouse
Applications: ELISA, Flow Cytometry, Immunocytochemistry, Immunohistochemistry
Size: 120Tests
Form: Supplied as a liquid in PBS, pH 7.2, 5% glycerol, 0.2% BSA, 0.05% sodium azide.
P type: Mab
Isotype: IgG1
Purity: Purified by Protein A affinity chromatography.
References: 1. R.Q. Hintzen, et al, (1994) J Immunol 152: 1762-1773. 2. M.R. Bowman, et al, (1994) J Immunol 152: 1756-1761. 3. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 1137-1138. 4. K. Agematsu, et al, (1995) J Immunol 154: 3627-3635.
Additional info: Recognizes the human CD70 molecule at ~30kD.

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