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CD62p (LECAM, P-Selectin, Endothelial Leukocyte Adhesion Molecule) (PE)

Cat no: C2415-55J

Supplier: United States Biological
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Human CD62P appears on the surface of activated endothelial cells or platelets and is likely involved with regulating the recruitment of leukocytes to areas of inflammation or infection. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:50. or use 1.6ul undiluted antibody per 5x10e5 cells per test Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: Platelet rich plasma from heparinized blood was activated with 1U/ml Thrombin in the presence of citrate for 10 minutes. Platelets were fixed in 1% buffered formaldehyde, washed twice and pre incubated 5 minutes with 20ul of human IgG at 250ug/ml (to block non specific binding) after which they were incubated 45 minutes on ice with 80ul of C2415-55J at a dilution factor of 1:50 (10ug/ml). Cells were washed three times, fixed and analyzed by FACS. A net 22% of the gated population stained positive with a mean shift of 1.18 log10 fluorescent units when compared to a mouse IgG1/R-PE negative control at a similar concentration. Binding was blocked when pre incubated 10 minutes with 20ul of 0.5 mg/ml C2415-55J. Storage and Stability: May be stored at 4 degrees C before opening. DO NOT FREEZE! Stable at 4 degrees C as an undiluted liquid. Dilute only prior to immediate use. Stable for at least 12 months at 4 degrees C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.
Catalogue number: C2415-55J
Reactivities: Human
Hosts: Mouse
Applications: Flow Cytometry
Size: 120Tests
Form: Supplied as a liquid in PBS, pH 7.2, 15% glycerol, 0.2% BSA, 0.04% sodium azide
P type: Mab
Isotype: IgG1
Purity: Purified from tissue culture supernatant by Protein A affinity chromatography
References: 1. G.I. Johnston, et al, (1989) Cell 56: 1033-1044. 2. J.G. Geng, et al, (1990) Nature 343: 757-760. 3. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford (1995) p. 1501-1501 4. M.R. Elstad, et al, (1995) J Immunol 155: 2109-2122.
Additional info: Recognizes the protease sensitive lectin epitope of CD62P. Blocks the activated endothelium or platelet-neutrophil interaction.

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