Human CD62L is as adhesion molecule found on most leukocytes and plays a primary role in mediating initial leukocyte interaction with activated vascular endothelium (1). Human CD62L has signal transduction functions where engagement leads to increased calcium levels and upregulated expression of certain integrins (2).
Suitable for use in Flow Cytometry and blocking CD62L function and induces expression of beta-1 and beta-2 integrins. Other applications not tested.
Flow Cytometry: 1:50 (80ul/test)
Optimal dilutions to be determined by the researcher.
1. Five x 10e5 cultured Raji cells were washed and incubated 45 minutes on ice with 80ul of C2415-15C at a 1:50 dilution factor (10 mg/ml).
2. Cells were washed 3 times, fixed and analyzed by FACS.
3. Cells stained positive with a mean shift of 1.24 log10 fluorescent units when compared to a Mouse IgG2a/FITC negative control at a similar concentration.
4. Binding was 99% blocked when cells were pre incubated 10 minutes with 20ul of 0.5mg/ml C2415-15A.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.