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CD50 (Intercellular Adhesion Molecule, ICAM-3, Lymphocyte Function-Associated Antigen-1, LFA-1) (Biotin)

CD50 (Intercellular Adhesion Molecule, ICAM-3, Lymphocyte Function-Associated Antigen-1, LFA-1) (Biotin)

Cat no: C2406-15A

Supplier: United States Biological
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CD50 is a 120-140 kD type I membrane protein also known as intercellular adhesion molecule 3 (ICAM-3). It is broadly expressed on leukocytes, endothelial cells and Langerhans cells. CD50 is a member of the Ig superfamily. It provides costimulatory signal for T cells and regulates cell adhesion through interaction with LFA-1 (CD11a/CD18, aL/b2) integrin. Applications: Suitable for use in Immunohistochemistry, Flow Cytometry and ELISA. Other applictions have not been tested. Recommended Dilution: Immunohistochemistry: 0.5-1.25ug/ml. Can be used to stain both indirectly or directly acetone-fixed cryostat sections or cell smears. Flow Cyotmetry: 1:20 for HL-60 cells. Can be used in a 2-step procedure using the biotin-(strept)avidin system. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: C2406-15A
Reactivities: Human
Hosts: Mouse
Applications: ELISA, Flow Cytometry, Immunohistochemistry
Conjugates: Biotin
Size: 100Tests
Form: Supplied as a liquid in 50mM Tris, 100mM sodium chloride, pH 7.3, 1% BSA, 0.02% sodium azide. Labeled with Biotin.
P type: Mab
Isotype: IgG1
Purity: Highly purified (SDS-PAGE)
Additional info: Recognizes human CD50 (ICAM-3). Does not crossreact with ICAM-1, ICAM-2 or LFA-1.

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