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Cat no: C2400-27D

Supplier: United States Biological
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Human CD45 is expressed on all cells of hematopoietic origin, except erythrocytes. CD45 is a transmembrane tyrosine phosphatase which can exist in at least nine different isoforms resulting from tissue-specific alternative RNA splicing of exons 4-7 of a single gene coding for the various N-terminal peptide segments. CD45 plays an essential role in lymphocyte activation. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. Five x 10e5 ficoll prepared peripheral blood lumophocytes were washed and preincubated 5 minutes with 250ug/ml human IgG (to block non-specific binding). 2. Cells were then incubated 45 minutes on ice with 80ul of C2400-27D at a dilution of 1:50. 3. Cells washed three times, fixed and analyzed by FACS. 4. Cells stained positive with a mean shift of 1.41 log10 fluorescent units when compared to a mouse IgM/FITC negative control (I1905-26E) at a similar concentration. 5. Binding was 55% blocked when cells were pre-incubated with 20ul of C2400-27B at 0.5mg/ml. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: C2400-27D
Reactivities: Human
Hosts: Mouse
Applications: Flow Cytometry
Size: 120Tests
Form: Supplied as a liquid in 500mM potassium chloride, 150mM sodium chloride, 5% glycerol, 0.2% BSA, 0.04% sodium azide. labeled with FITC.
P type: Mab
Isotype: IgM
Purity: ~95% pure by SDS-PAGE. Purified Immunoglobin.
References: 1. L.J. Murray, et al, Clin Exp Immunol 59:315-326 (1985). 2. I.S. Trowbridge & M.L. Thomas, Annu Rev Immunol 12:85-116 (1994). 3. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, 394-397 (1995). 4. D. Leitenberg, et al, J Exp Med 183: 249-259 (1996).
Additional info: Recognizes thuman CD45R isoforms at ~200-220kD.

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