Human CD45 is expressed on all cells of hematopoietic origin, except erythrocytes. CD45 is a transmembrane tyrosine phosphatase which can exist in at least nine different isoforms resulting from tissue-specific alternative RNA splicing of exons 4-7 of a single gene coding for the various N-terminal peptide segments. CD45 plays an essential role in lymphocyte activation.
Applications:
Suitable for use in Flow Cytometry. Other applications not tested.
Recommended Dilution:
Optimal dilutions to be determined by the researcher.
Flow Cytometry Protocol:
1. Five x 10e5 ficoll prepared peripheral blood lumophocytes were washed and preincubated 5 minutes with 250ug/ml human IgG (to block non-specific binding).
2. Cells were then incubated 45 minutes on ice with 80ul of C2400-27D at a dilution of 1:50.
3. Cells washed three times, fixed and analyzed by FACS.
4. Cells stained positive with a mean shift of 1.41 log10 fluorescent units when compared to a mouse IgM/FITC negative control (I1905-26E) at a similar concentration.
5. Binding was 55% blocked when cells were pre-incubated with 20ul of C2400-27B at 0.5mg/ml.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.