Human CD45 is expressed on all cells of hematopoietic origin, except erythrocytes. CD45 is a transmembrane tyrosine phosphatase which can exist in at least nine different isoforms resulting from tissue-specific alternative RNA splicing of exons 4-7 of a single gene coding for the various N-terminal peptide segments. CD45 plays an essential role in lymphocyte activation.
Suitable for use in Flow Cytometry. Other applications not tested.
Optimal dilutions to be determined by the researcher.
Flow Cytometry Protocol:
1. Five x 10e5 ficoll prepared peripheral blood lumophocytes were washed and preincubated 5 minutes with 250ug/ml human IgG (to block non-specific binding).
2. Cells were then incubated 45 minutes on ice with 80ul of C2400-27D at a dilution of 1:50.
3. Cells washed three times, fixed and analyzed by FACS.
4. Cells stained positive with a mean shift of 1.41 log10 fluorescent units when compared to a mouse IgM/FITC negative control (I1905-26E) at a similar concentration.
5. Binding was 55% blocked when cells were pre-incubated with 20ul of C2400-27B at 0.5mg/ml.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.