Recommended concentration for the unlabeled antibody is 2ug/ml and a 1:20 dilution of the labeled antibodies, as determined on HACAT cells.
Immunohistochemistry (frozen sections, paraffin sections):
Can be used to stain both indirectly or directly acetone-fixed cryostat sections or cell smears. Recommended is the alkaline phosphatase-anti-alkaline-phosphatase (APAAP), or peroxidase anti-peroxidase (PAP) procedures or the three stage immunoperoxidase technique on acetone-fixed cryostat sections. It may be used at a concentration of 2-10ug/ml for cryostat sections. Suitable for paraffin-embedded tissue after microwave treatment.
Western Blot: Recommended concentration: 2-10ug/ml.
Optimal conditions must be determined individually for each application.
Long Term Storage:
+4 degrees C (Biotin- and FITC-conjugated antibodies)