CD4, a single chain transmembrane glycoprotein, is found on a T cell subset (helper/inducer) representing 45% of peripheral blood lymphocytes. It is also present on 80% of thymocytes and at a lower level on monocytes. It is involved in recognition of antigen presented along with MHC class II by APCs. It serves as receptor for HIV.
Applications:
Suitable for use in Flow Cytometry. Other applications not tested.
Recommended Dilutions:
Flow Cytometry: Neat, use 10ul to label 10e6 cells in 100ul.
Optimal dilutions to be determined by the researcher.
Recommended Negative Control:
I1904-84B2: IgG2a NEGATIVE CONTROL, Murine (PE)
Hybridoma:
NS1 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability:
Lyophilized powder may be stored at 4 degrees C. Reconstitute by adding sterile ddH2O. Aliquot and store at 4 degrees C. DO NOT FREEZE! Reconstituted product is stable for 12 months. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.