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CD4 (CD4 Receptor, CD4mut, T Cell Surface Antigen T4/Leu3, T Cell Surface Glycoprotein CD4) (PE)

Cat no: C2255-01U

Supplier: United States Biological
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CD4, a single chain transmembrane glycoprotein, is found on a T cell subset (helper/inducer) representing 45% of peripheral blood lymphocytes. It is also present on 80% of thymocytes and at a lower level on monocytes. It is involved in recognition of antigen presented along with MHC class II by APCs. It serves as receptor for HIV. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: Neat, use 10ul to label 10e6 cells in 100ul. Optimal dilutions to be determined by the researcher. Recommended Negative Control: I1904-84B2: IgG2a NEGATIVE CONTROL, Murine (PE) Hybridoma: NS1 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: Lyophilized powder may be stored at 4 degrees C. Reconstitute by adding sterile ddH2O. Aliquot and store at 4 degrees C. DO NOT FREEZE! Reconstituted product is stable for 12 months. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Catalogue number: C2255-01U
Reactivities: Bovine
Hosts: Mouse
Applications: Flow Cytometry
Size: 100Tests (1ml)
Form: Supplied as a lyophilized powder from PBS, 5% sucrose, 1% BSA, 0.09% sodium azide. Labeled with R-Phycoerythrin (PE). Reconstitute in 1ml sterile ddH2O.
P type: Mab
Isotype: IgG2a
Purity: Purified by Protein G affinity chromatography.
References: 1. Bensaid, A. et al. (1991) Individual antigens of cattle. Bovine CD4 (BoCD4). Vet. Immunol. Immunopathol. 27: 51-54. 2. Eskra, L. et al. (1991) Effect of monoclonal antibodies on in vitro function of T-cell subsets. Vet. Immunol. Immunopathol. 27: 215-231.
Additional info: Recognizes bovine CD4. Precipitates a molecule of ~50kD and is considered to be the bovine homologue of Human CD4. The phenotype, tissue distribution and function of T-cells expressing the bovine CD4 antigen are similar to those in other species. Expression on macrophages has not yet been detected.

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