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CD3 (Early T Cell Marker) (APC)

CD3 (Early T Cell Marker) (APC)

Cat no: C2254-01E

Supplier: United States Biological
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The human CD3/T cell receptor (TcR) complex is made up of at least five CD3 proteins (g, d, e , h, z) in association with either a/b or g/d proteins of the TcR. The TcR recognizes antigens in association with MHC molecules after which protein chains of the CD3 complex mediate activation signals triggered by TcR antigen binding. CD3 is expressed on greater than 95% of circulating human peripheral T cells. Functional Application: Antibody will activate T cells expressing CD3e. Applications: Suitable for use in Flow Cytometry, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C before opening. DO NOT FREEZE! Stable at 4 degrees C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Freezing APC conjugates will result in a substantial loss of activity. APC conjugates are sensitive to light.
Catalogue number: C2254-01E
Reactivities: Human
Hosts: Mouse
Applications: Flow Cytometry, Immunohistochemistry, Western Blot
Size: 120Tests
Form: Supplied as a liquid in PBS, pH 7.2, 15% glycerol, 0.2% BSA, 0.05% sodium azide. Labeled with allophycocyanin.
P type: Mab
Isotype: IgG1
Purity: Purified by Protein A affinity chromatography.
References: 1. P.C. L. Beverly & R.E. Callard, Eur J Immunol (1981) 11: 329-334. 2. Leukocyte Typing IV (W. Knapp, et al, eds.) Oxford University Press, Oxford, (1989) p. 3. 290-314. A. Salmeron, et al, (1991) J Immunol 147 : 3047-3052. 4. G. Thibault & P. Bardos, (1995) J Immunol 154: 3814-3820.
Additional info: Recognizes the 20kD epsilon chain of the CD3 molecule complex. Antibody will activate T cells expressing CD3e.

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