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CD279 (Programmed cell death-1, PD-1) (PE)

Cat no: P3120-62B

Supplier: United States Biological
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CD279 is a co-stimulatory molecule also known as programmed cell death-1 (PD-1). CD279 is 50-55kD membrane protein which is a member of the CD28 family, and functions mainly as a negative regulator of T-cell activation. CD279 has two specific ligands; CD274 (PD-L1) and CD273 (PD-L2), and their interaction is key in the balance between stimulatory and inhibitory signals needed for effective immune responses to microbes and self-tolerance. CD279 is inducibly expressed by T-cells, B-cells, NK-T-cells and monocytes upon activation. Loss of CD279 function has been associated with a number of autoimmune diseases, including rheumatoid arthritis, type I diabetes and ankylosing spondylitis. Recent studies suggest that CD279 could be targeted therapeutically in the treatment of HIV infection to reduce T-cell exhaustion. Applications: Suitable for use in Flow Cytometry. Other applications have not been tested. Recommended Dilutions: Flow Cytometry: Neat; Use 10ul to label 1x10e6 cells in 100ul Optimal dilutions to be determined by the researcher. Hybridoma: Spleen cells from immunized C3H mice were fused with cells of the P3U1 myeloma cell line. Recommended Negative Control: I1904-79D: IgG1 Murine Negative Control (PE) Storage and Stability: Lyophilized powder may be stored at 4 degrees C. Reconstitute by adding sterile ddH2O. Aliquot and store at 4 degrees C. DO NOT FREEZE! Reconstituted product is stable for 12 months. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: P3120-62B
Reactivities: Human
Hosts: Mouse
Applications: Flow Cytometry
Size: 100Tests
Form: Supplied as a lyophilized powder from PBS, pH7.4, 1% BSA, 0.09% sodium azide, 5% sucrose. Labeled with R-Phycoerythrin (PE). Reconstitute with 1ml sterile ddH2O.
P type: Mab
Isotype: IgG1
Purity: Purified by Protein G affinity chromatography.
Alternative names: PD-1
References: 1. Kanai, T. et al. (2003) Blockade of B7-H1 suppresses the development of chronic intestinal inflammation. J. Immunol. 171: 4156-4163. 2. Freeman, G.J. et al. (2006) Reinvigorating exhausted HIV-specific T cells via PD-1-PD-1 ligand blockade. J. Exp. Med. 203: 2223-2227. 3. Keir, M.E. et al. (2007) PD-1 and its ligands in T-cell immunity. Curr. Opin. Immunol. 19: 309-314.
Additional info: Recognizes human CD279.

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