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Cat no: C2268-50A

Supplier: United States Biological
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Antibodies to human CD17 react with lactosylceramide which is expressed on granulocytes, monocytes, platelets, and basophils (1). CD17 expression is downmodulated on activated granulocytes. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 1:50 (5ug/ml); 80ul labels 5x10e5 cultured Nalm-6 cells. Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. 5x10e5 cultured Nalm-6 cells were washed and incubated 45 minutes on ice with 80ul of C2268-50A at a 1:50 (5ug/ml) dilution. 2. Cells were then washed three times, fixed and analyzed by FACS. 3. Cells stained positive with a mean shift of 1.41 log10 fluorescent units when compared to a Mouse IgM/FITC negative control at a similar concentration. 4. Binding was blocked when cells were pre incubated 10 minutes with 20ul of 0.5mg/ml anti-CD60b antibody (C2268-50). Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: C2268-50A
Reactivities: Human
Hosts: Mouse
Applications: Flow Cytometry
Size: 120Tests
Form: Supplied as a liquid in PBS, pH 7.5, 0.2% BSA, 0.04% sodium azide, 5% glycerol. Labeled with fluorescein isothiocyanate (FITC).
P type: Mab
Isotype: IgM,k
Purity: Purified
References: 1. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford (1995) p. 822-823. 2. Nakayama H, K Iwabuchi, et al. (2008) J Leukocyte Biol 83:728-741.
Additional info: Recognizes human CD17 on myeloid cells.

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