Antibodies to human CD17 react with lactosylceramide which is expressed on granulocytes, monocytes, platelets, and basophils (1). CD17 expression is downmodulated on activated granulocytes.
Suitable for use in Flow Cytometry. Other applications not tested.
Flow Cytometry: 1:50 (5ug/ml); 80ul labels 5x10e5 cultured Nalm-6 cells.
Optimal dilutions to be determined by the researcher.
Flow Cytometry Protocol:
1. 5x10e5 cultured Nalm-6 cells were washed and incubated 45 minutes on ice with 80ul of C2268-50A at a 1:50 (5ug/ml) dilution.
2. Cells were then washed three times, fixed and analyzed by FACS.
3. Cells stained positive with a mean shift of 1.41 log10 fluorescent units when compared to a Mouse IgM/FITC negative control at a similar concentration.
4. Binding was blocked when cells were pre incubated 10 minutes with 20ul of 0.5mg/ml anti-CD60b antibody (C2268-50).
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.