Antibodies to human CD17 react with lactosylceramide which is expressed on granulocytes, monocytes, platelets, and basophils (1). CD17 expression is downmodulated on activated granulocytes.
Applications:
Suitable for use in Flow Cytometry. Other applications not tested.
Recommended Dilutions:
Flow Cytometry: 1:50 (5ug/ml); 80ul labels 5x10e5 cultured Nalm-6 cells.
Optimal dilutions to be determined by the researcher.
Flow Cytometry Protocol:
1. 5x10e5 cultured Nalm-6 cells were washed and incubated 45 minutes on ice with 80ul of C2268-50A at a 1:50 (5ug/ml) dilution.
2. Cells were then washed three times, fixed and analyzed by FACS.
3. Cells stained positive with a mean shift of 1.41 log10 fluorescent units when compared to a Mouse IgM/FITC negative control at a similar concentration.
4. Binding was blocked when cells were pre incubated 10 minutes with 20ul of 0.5mg/ml anti-CD60b antibody (C2268-50).
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.