The lymphocyte Fc gamma receptors recognize the Fc portion of IgG, presented either as immune complexes or as free antibody. The different classes of receptors are distinct because of varying size, tissue distribution and affinity for IgGs. The Fc type II receptor is expressed on a wide variety of cells including B cells, hematopoietic cells, monocyte/macrophages, neutrophils, platelets, Langerhans cells, eosinophils, basophils, trophoblasts, and endothelial cells of the placenta. The Fc gamma type III receptors are higher affinity than the type II and are expressed on macrophages, NK cells and neutrophils. Both types of receptors can be expressed on the same cell and in varying ratios. The receptors are constitutively expressed, although cytokines and lymphokines can modulate their expression. Besides identifying Fc gamma R+ cells, monoclonal antibodies to the Fc gamma II/III receptor have been used to block Fc receptor binding of IgG, Fc mediated signal transduction and effector functions, clearance of immune complexes and to attenuate infection by organisms dependent on Fc gamma R for parasitic invasion.
Suitable for use in Neutralization, Flow Cytometry, Western Blot and Immunoprecipitation. Other applications not tested.
Neutralization: Blocks binding of human IgG to Fc(gamma)RIII.
Flow Cytometry: 5-10ug/ml
Western Blot: 1:1000 Use under non-reducing conditions.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.