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CD16 (Fc gamma RIII alpha, NK cells) (FITC)

CD16 (Fc gamma RIII alpha, NK cells) (FITC)

Cat no: C2267-33C

Supplier: United States Biological
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Human CD16 is a low affinity receptor for complexed IgG and occurs in two distinct forms, as a glycosyl phosphtidyl inositol (GPI) anchored protein in granulocytes, and as an integral membrane protein on NK cells and macrophages. Functional Application: Antibody blocks binding of complexed IgG to CD16 (2, 5). Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: C2267-33C
Reactivities: Human
Hosts: Mouse
Applications: Flow Cytometry
Size: 120Tests
Form: Supplied as a liquid in PBS, pH 7.2, 5% glycerol, 0.2% BSA, 0.05% sodium azide.
P type: Mab
Isotype: IgG1
Purity: Purified by Protein A affinity chromatography.
References: 1. H.B. Fleit, et al, (1982) Proc Natl Acad Sci USA 79: 3275-3279. 2. Leukocyte Typing IV (W. Knapp, et al, eds.) Oxford University Press, Oxford, (1989) p. 574-597. 3. G. Trinchieri & N. Valiante, (1993) Natural Immunity 12: 218-234. 4. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 805-814. 5. J.C. Edberg & R.P. Kimberly, (1997) J Immunol 159: 3849-3857.
Additional info: Recognizes both allelic forms of CD16. Antibody blocks binding of complexed IgG to CD16.

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