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CD14 (FITC)

Cat no: C2265-33R


Supplier: United States Biological
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Human CD14 is strongly expressed on myelomonocyte lineage cells including monocytes, macrophages, and a subset of granulocytes. CD14 is a high affinity receptor for complexes of lipopolysaccharide and lipopolysaccharide binding protein. A soluble form of CD14 also binds to LPS and LBP. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: C2265-33R
Reactivities: Human
Hosts: Mouse
Applications: Flow Cytometry
Size: 120Tests
Form: Supplied as a liquid in PBS, pH 7.2, 5% glycerol, 0.2% BSA, 0.05% sodium azide.
P type: Mab
Isotype: IgG2a
Purity: Purified by Protein A affinity chromatography.
References: 1. N. Hogg, et al, (1984) Immunology 53: 753-767. 2. M.O. Labeta, et al, (1993) Eur J Immunol 23: 2144-2151. 3. Leukocyte Typing IV (W. Knapp, et al, eds.) Oxford University Press, Oxford, (1989) 4. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 778-788. 5. S.D. Wright, (1995) J Immunol 155: 6-8.
Additional info: Recognizes a membrane bond CD14 molecule of about 53kD. Antibody will also immunoprecipitate a soluble form of CD14.

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