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CD134 Ligand (CD134L, OX40L, CD252) (PE)

Cat no: C2515-40D

Supplier: United States Biological
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Human CD134L is a type II membrane protein with homology to TNF which is expressed on activated B cells and activated endothelial cells. CD134L binds to CD134 present on activated T cells, providing a costimulatory signal (1). This pathway seems to be more effective for costimulation of CD4+ helper T cells than for CD8+ effector T cells (4). Blockade of this interaction in mouse abrogated immunological effects in several models of immflamation and rejection (5,6,7,8). Applications: Suitable for use in Flow Cytometry and ELISA. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:50 labels 5x10e5 HUVEC cells Optimal dilutions to be determined by the researcher. FACS Protocol: 1. Cultured human umbilical cord vein endothelial cells (HUVEC) were washed two times. 2. Five x 10e5 cells per tube were incubated 45 minutes on ice with 80ul of C2515-40D at 10ug/ml. 3. Cells were washed three times, fixed and analyzed by FACS. 4. A net 56% sub population of the cells stained positive with a mean shift 0.95log10 fluorescent units when compared to a Mouse IgG1/R-PE at a similar concentration. 5. Binding was 86% blocked when cells were pre-blocked 10 min with 20ul C2515-40A. Storage and Stability: May be stored at 4 degrees C before opening. DO NOT FREEZE! Stable at 4 degrees C as an undiluted liquid. Dilute only prior to immediate use. Stable for at least 12 months at 4 degrees C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.
Catalogue number: C2515-40D
Reactivities: Human
Hosts: Mouse
Applications: ELISA, Flow Cytometry
Size: 120Tests
Form: Supplied as a liquid in 50mM Sodium Phosphate, pH 7.5, 500mM Potassium Chloride, 150mM sodium chloride, 15% Glycerol, 0.2% BSA, 0.04% sodium azide.
P type: Mab
Isotype: IgG1,k
Purity: Purified by Protein A affinity chromatography.
References: 1. W. R. Godfrey, et al, (1994) J Exp Med 180: 757-763. 2. L.M. Higgins, et al, (1999) J Immunol 162: 486-493. 3. A.D. Weinberg, et al, (1999) J Immunol 162: 1818-1826. 4. V.Y. Taraban, et al, (2002) Eur J. Immunol. 32: 3617-3627. 5. V. Malmstrom, et al, (2001) J Immunol 166: 6972. 6. C. Nohara, et al, (2001) J Immunol 166: 2108-2115. 7. X. Yuan, et al, (2003) J Immunol 170: 2949-2955. 8. L. Tian, et al, (2002) Transplantation 74(1): 133-138
Additional info: Recognizes the human CD134 ligand. Blocks binding of recombinant CD134-muIg.

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