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CD117 (c-kit, Stem Cell Factor, SCF Receptor, Steel Factor Receptor, Mast Cell Growth Factor, MGF) (PE)

Cat no: C2448-03K

Supplier: United States Biological
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Human CD117 (c-kit) is a transmembrane protein with receptor tyrosine kinase capacity and serves as the receptor for steel factor (SLF). CD117 is expressed on almost all hematopoietic progenitor cells and receptor/ligand interaction is crucial for development of hematopoietic stem cells. Applications: Suitable for use in Flow Cytometry. Does not block binding of SCF. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:50, 80ul/test Optimal dilutions to be determined by the researcher. Protocol: Five x 10e5 cultured HEL human tumor cells were washed and incubated 45 minutes on ice with 80ul of C2448-03K at a 1:50 dilution. Cells were washed three times, fixed and analyzed. Cells stained positive with a mean shift of 1.4 log10 fluorescent units when compared to a mouse IgG1/PE negative control at a similar concentration. Binding was 93% blocked when cells were pre incubated 10 minutes with 20ul of 0.5mg/ml C2448-03K. Storage and Stability: May be stored at 4 degrees C before opening. DO NOT FREEZE! Reconstitute with 1ml sterile dH2O. Stable at 4 degrees C as an undiluted liquid. Dilute only prior to immediate use. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. Protect from light.
Catalogue number: C2448-03K
Reactivities: Human
Hosts: Mouse
Applications: Flow Cytometry
Size: 120Tests
Form: Supplied as a liquid in PBS, 0.2% BSA, 0.04% sodium azide, 15% glycerol. Labeled with PE.
P type: Mab
Isotype: IgG1
Purity: Purified by Protein A affinity chromatography.
References: 1. L.K. Ashman, et al, (1994) J Cellular Physiol 158: 545-554. 2. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 1856, 1882-1888. 3. .R. Keller, et al, (1995) Blood 86: 1757-1764.
Additional info: Recognizes the human CD117 molecule of ~150kD.

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