CD105 (Endoglin, gp160, TGF beta1,3 Receptor, HHT1, ORW1, Osler Rendu Weber syndrome 1) (FITC)

CD105 is a disulfide-linked homodimeric type I transmembrane glycoprotein (1, 2). Two isoforms of CD105, a short form and a long form, differ by 33aa in their cytoplasmic tails (3). The long form (180kD in size) is considered the predominant form of the protein although both isoforms have been detected by RT-PCR in endothelial cells, monocytic cell lines, and placenta (3, 4). CD105 serves as the regulatory component of the TGF-beta receptor complex by associating with TGF-b receptor I and II (5, 6). These receptor complexes efficiently bind TGF-b1 and TGF-b3 with high affinity (5, 7). CD105 also binds other members of the TGF-b superfamily including activin-A, BMP-7 and BMP-2 (5). Functionally, CD105 plays an important role in angiogenesis (7, 8), hematopoiesis (9, 10) and cardiac development (9). CD105 was also shown to be a functional marker that defines long-term repopulating hematopoietic stem cells from the bone marrow "side-population" cells (11). Multipotent stem cells have been isolated from bone marrow and muscle tissue based on their expression of CD105 (12, 13). Clinically, mutations in the gene for CD105 result in the autosomal dominant disorder Hereditary Hemorrhagic Telangiectasia Type 1 (14). In addition, soluble levels of shed CD105 have been associated with poor prognosis in breast, colorectal and other types of cancer (15, 16). Further investigations may elucidate the role of CD105 and its ligands in a variety of biologic processes. Applications: Suitable for use in Flow Cytometry and Immunohistochemistry. Will augment binding of TGF-b1 to CD105 expressing leukemia cells. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:50 Immunohistochemistry: Frozen Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.