Carbonic anhydrase (CA) catalyzes the reversible reaction of CO2 + H2O = HCO3- + H+ , which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption. CA9, also known as membrane antigen MN and renal cell carcinoma (RCC)-associated antigen G250, is a transmembrane enzyme expressed primarily in carcinoma cells. It is one of the best markers for hypoxia and for RCC.
Suitable for use in Flow Cytometry. Other applications not tested.
Flow cytometry: 10ul labels 1x10e6 cells
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C before opening. DO NOT FREEZE! Stable at 4 degrees C as an undiluted liquid. Stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Freezing Allophycocyanin conjugates will result in a substantial loss of enzymatic activity.