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Calcitonin, Human, BioAssay(TM) ELISA Kit, Highly Sensitive

Cat no: C0115-12

Supplier: United States Biological
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This Human Calcitonin ELISA Kit is based on a competitive immunoassay format. The antigen is captured by antibodies coated on a 96-well plate. A constant concentration of Bt-tracer (biotinylated tracer) and varying concentrations of unlabeled standard or sample peptide compete for binding specifically to the antiserum. Captured Bt-tracer is subsequently bound by SA-HRP (streptavidin-conjugated horseradishperoxidase), which produces a soluble colored or fluorescent product after a substrate is added. The calcitonin standard is used to make a standard curve in the range 0-25 ng/ml. Standard curves are S-shaped (on a semi-log plot). The measuring range is the range of standard concentrations near the middle or near the IC50 of the standard curve. Unknown sample concentrations are measured by comparing their absorbance with the standard curve. Sample Type: Formulated for extracted samples Assay Range: 0.02-25ng/ml Average IC50: 1ng/ml Kit Components: C0115-12A: Microtiter Plate, 1x96 wells C0115-12B: EIA Buffer Concentrate (20X), 1x50ml C0115-12C: Antiserum, lyophilized powder. C0115-12D: Standard, 1x1ug. Reconstitute with EIA buffer. H-Cys-Gly-Asn-Leu-Ser-Thr-Cys-Met-Leu-Gly-Thr-Tyr-Thr-Gln-Asp-Phe-Asn-Lys-Phe-His-Thr-Phe-Pro-Gln-Thr-Ala-Ile-Gly-Val-Gly-Ala-Pro-NH2, (Disulfide bond) C0115-12E: Biotinylated Tracer, lyophilized powder C0115-12F: Streptavidin-HRP Concentrate (200X). 1x100ul C0115-12G: TMB Substrate Solution, 1x11ml. Mixture of TMB and H2O2 C0115-12H: Stop Solution, 1x15ml (2N HCl) Storage and Stability: Store C0115-12C (Antiserum) and C0115-12D (Standard) at -20 degrees C. Store all the other kit components at 4 degrees C. Kit is stable for up to 6 months. Cross-reactivity: Peptide Percent (%) Calcitonin (human) 100 Calcitonin (rat) 5.4 Calcitonin C-terminal adjacent Peptide (rat) 0.001 Calcitonin (eel) 0 Calcitonin (salmon I) 0 Calcitonin (chicken) 0 Amylin (human) 0 Alpha-CGRP (human) 0
Catalogue number: C0115-12
Size: 1Kit
References: 1. T. Porstmann and S.T. Kiessig Enzyme immunoassay techniques. An Overview. J. Immunol. Methods 150, 5-21 (1992). 2. S. Avrameas Amplification systems in immunoenzymatic techniques. J. Immunol. Methods 150, 23-32 (1992). 3. E. Bucht et al. A rapid extraction method for serum calcitonin. Clin. Chim. Acta, 195, 115-124 (1991).

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