Human BAFF (for B cell activating factor belonging to the TNF family) is a newly discovered novel ligand of the TNF family. BAFF plays an important role as costimulator of B cell proliferation and function. Recombinant Human BAFF is a soluble 17kD protein containing 153 amino acid residues, comprising the TNF-like extra-celluar domain of BAFF.
Biological Activity:
Assay#1: Determined by the dose-dependent stimulation of IL-8 production by Human PBMC (Peripheral Blood Mononuclear Cells). The ED50 for this effect is 5-10ng/ml. Please Note: Results may vary with PBMC donors.
Assay#2: Determined by a mouse splenocyte survival assay. The expected ED50 for this effect is 0.5-2.0ug/ml.
Storage and Stability:
Lyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile dH2O and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Country of Origin: USA
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