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b2-Microglobulin (B2M) BioAssay(TM) ELISA Kit

Cat no: M5891

Supplier: United States Biological
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Human Beta-2 Microglobulin (B2M) is a small (11kD) polypeptide that forms the invariant subunit of the class I HLA-antigens (Human Lymphocyte Antigen) on the cell membrane (1). Increased concentrations of B2M in serum have been found in patients with renal diseases, malignancies (especially of lymphatic origin) and infectious diseases, e.g., AIDS. B2M is the most powerful single prognostic variable in multiple myelome, it is also a good predictor for acute rejection in renal transplantation, furthermore the measurement of B2M is very useful for detecting and monitoring the changes in the glomerular filtration rate in diabetic nephropathy. Sensitivity: ~0.5ng/ml Assay Time: 90 min. Kit Components: M5891-01: Microtiter Plate 1x96wells M5891-02: Sample Diluent 1x50ml M5891-03: Standard 2.5ng/ml 1x1ml M5891-04: Standard 5ng/ml 1x1ml M5891-05: Standard 20ng/ml 1x1ml M5891-06: Standard 50ng/ml 1x1ml M5891-07: Standard 150ng/ml 1x1ml M5891-08 :B2M (HRP) 1x11ml M5891-09: Wash Buffer (20X) 1x50ml M5891-10: HRP Substrate A 1x11ml M5891-11: HRP Substrate B 1x11ml M5891-12: Stop Solution 1x10ml Storage and Stability: Store at components except standards at 4 degrees C. Store opened Standards M5891-03-M5891-07 as aliquots at -20 degrees C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: M5891
Reactivities: Human
Applications: ELISA
Size: 1Kit
References: 1. Viedma, J.A., et al., Clin. Chem. 12: 2464 (1992). 2. Bernard, A.M., Clin. Chim. Acta 126: 1 (1982). 3. Tienhara, A., Clin. Chem. 36: 1961 (1990). 4. Hemmingsen, L., Clin. Lab Invest. 45: 367 (1985). 5. Bjerrum, O.W., Clin. Chim. Acta 155: 69 (1986).

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