Atg7 (Autophagy Protein 7, Ubiquitin-activating enzyme E1-like protein, hAGP7, APG7-like, ATG7, APG7L)

Functions as an E1 enzyme essential for multisubstrates such as GABARAPL1 and ATG12. Forms intermediate conjugates with GABARAPL1 (GABARAPL2, GABARAP or MAP1ALC3). Formation of the final GABARAPL1-PE conjugate is essential for autophagy. Subunit: Homodimer (By similarity). Interacts with ATG3 and ATG12. The complex, composed of ATG3 and ATG7, plays a role in the conjugation of ATG12 to ATG5. In yeast, ATG7 appears to be required for fusion of peroxisomal and vaculuolar membranes. Belongs to the ATG7 family. \n\nDomain:\nThe C-terminal part of the protein is essential for the dimerization and interaction with ATG3 and ATG12. \n\nTissue specificity: \nWidely expressed, especially in kidney, liver, lymph nodes and bone marrow.\n\nApplications:\nSuitable for use in Immunofluorescence and Immunohistochemistry. Other applications have not been tested.\n\nRecommended Dilutions: \nImmunohistochemistry: 1:200-1:3000\nImmunofluorescence: 1:200-1:3000\nOptimal dilutions to be determined by the researcher. .\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 6 months at -20 degrees C. Reconstitute by adding sterile 40-50% glycerol, ddH2O. Aliquot and store at -20 degrees C. Reconstituted product is stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer

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SPECIFICATIONS

Catalog Number

A2298-74R

Size

100ul

Applications

IF, IHC

Hosts

Rabbit

Reactivities

Hum, Rat

Form

Supplied as a lyophilized powder. Reconstitute with 100ul sterile ddH2O.

P Type

Pab

Purity

Serum

Isotype

IgG

References

1. Yuan W, et al. Mol. Biol. Cell 10:1353-1366(1999). 2. Tanida I, et al. Biochem. Biophys. Res. Commun. 292:256-262(2002). 3. Tanida I, et al. J. Biol. Chem. 277:13739-13744(2002).4. Mizushima N, et al. Nature 395:395-398(1998). 5. Johnston M, et al. Science 265:2077-2082(1994). 6. Harding T.M, et al. J. Cell Biol. 131:591-602(1995). 7. Kim J, et al. Mol. Biol. Cell 10:1337-1351(1999). 8. Komatsu M, et al. J. Biol. Chem. 276:9846-9854(2001). 9. Klionsky D.J, et al. Dev. Cell 5:539-545(2003). 10. Chimura Y, et al. J. Biol. Chem. 279:40584-40592(2004).

Additional Info

Recognizes human ATG7. Species crossreactivity: rat. Species sequence homology: mouse, rat, S. cerevisiae, Macaca mulatta, and Canis familiaris.