Kit Components:
A3550-06A Arginine, symmetric, dimethyl (SYM10) 1x25ul (Human, mouse) (WB, IP, ICC)
Serum
Supplied as a liquid in 0.05% sodium azide, 30% glycerol.
Synthetic peptide corresponding to four symmetrical dimethyl-arginine-glycine repeats (KR*GR*GR*GR*G (which R* denotes symmetric dimethylation of arginine) (KLH).
A3550-06B Arginine, symmetric, dimethyl (SYM11) 1x25ug (Human) (WB)
Purified by Protein A affinity chromatography.
Supplied as a liquid in 0.1M Tris glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide), 30% glycerol.
Concentration: 1mg/ml
Synthetic peptide corresponding to KAAILLAQVAAR*GR*GR*GMGR*G (which R* denotes symmetric dimethylation of arginine) (KLH).
*When using A3550-06B as primary antibody, add 0.05% Twenn-20 to blocking buffer in steps 2. 3 and 5.
A3550-06C Arginine, symmetric, dimethyl (ASYM24) 1x25ul (Human, mouse) (WB, IP, ICC)
Serum
Supplied as a liquid in PBS, 0.05% sodium azide, 30% glycerol.
Synthetic peptide corresponding to KGR*GR*GR*GR*GPPPPPR*GR*GR*GR*G (which R* denotes asymmetric dimethylation of arginine) (KLH).
Positive Control (Included):
J9000 Jurkat Cell Lysate. 1x100ul Supplied as a liquid in modified RIPA buffer diluted with non-reducing sample buffer.
Concentration: 1mg/ml
Add 2.5ul of 2-mercaptoethanol/100ul of lysate and boil for 5 minutes to reduce the preparation. Load 20ug of reduced lysate per lane for minigels.
Applications:
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilutions:
A3550-06A: 1:500-1:1000
A3550-06B: 1-2ug/ml
A3550-06C: 1:500-1:1000
Storage and Stability:
Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Antibody aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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