Aprotinin, also called bovine pancreatic trypsin inhibitor, is a single chain globular Kunitz-type serine proteinase inhibitor found in bovine pancreas, parotid gland and lung. It forms stable, enzymatically inactive complexes with trypsin, chymotrypsin, plasmin and kallikrein. Aprotinin has been used therapeutically as an antifibrinolytic agent under the name Trasylol (Bayer) to reduce blood loss during cardiopulmonary bypass surgery but this use has been associated with an increased frequency of acute renal injury (1).
Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution:
ELISA: 1:33,000. 1ug/ml of A2300-01 (as capture antibody) forms an antibody pair with A2300-01B (as biotinylated detection antibody) for sandwich ELISA of aprotinin at sample concentrations of 4-250ug/ml. Both urine and plasma samples diluted 1:1000 can be assayed against standards in buffer. PBS-based buffers are unsuitable for this assay and 0.2M glycine sodium buffer, pH 8.8, is recommended for washing and dilution buffers.
Optimal dilutions to be determined by the researcher.
Culture Medium:
RPMI 1640 with 2-10% fetal calf serum
Hybridoma:
Sp2mIL6 myeloma cells with spleen cells from NMRI x Balb/c mice.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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