Human apo-SAA is a 104 amino acid polypeptide that circulates primarily in association with high-density lipoproteins (HDL). The level of apo-SAA, normally 1-5ug/ml in plasma, increases 500-1000 fold within 24 hours of an inflammatory stimulus and, under these conditions, is the most abundant HDL apo-lipoprotein. The human SAA gene codes for a 122 amino acid polypeptide, which contains an 18 amino acid N-terminal signal sequence.
Endotoxin: (same/less than)0.1ng/ug
For most in vitro applications, apo-SAA exerts its biological activity in the concentration range of 10-50ug/ml.
Storage and Stability:
Lyophilized powder may be stored at 4 degrees C for short-term only. Stable for at least 6 months at -20 degrees C. Reconstitute in 0.1% acetic acid to a concentration of 1ug/ul, aliquot and store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.