ABCG2, also called MXR, BCRP or ABCP, is highly overexpressed in several drug-resistant cell lines and localizes predominantly to the plasma membrane (Rocchi, E. et al., 2000, Biochem. Biophys. Res. Commun. 271:42-46). ABCG2 has also been shown to be expressed at relatively high levels in human hematopoietic stem cells (CD34+, CD38-) and to drop sharply in committed progenitors (CD34+/CD38+) (Scharenberg, C.W. et al., 2002, Blood 99:507-512).
Suitable for use in Flow Cytometry. Other applications not tested.
Flow Cytometry: Designed to quantitatively determine the percentage of cells bearing Bcrp1/ABCG2 on their cell surface within a population and qualitatively determine the density of this receptor on cell surfaces by flow cytometry. Washed cells are incubated with the PE-labeled monoclonal antibody which binds to the cells expressing the Bcrp1/ABCG2 structure. Unbound PE-conjugated antibody is then washed from the cells. Cells expressing Bcrp1/ABCG2 become fluorescently stained, with the intensity of staining directly proportional to the density of the
Bcrp1/ABCG2. Cell surface expression of Bcrp1/ABCG2 is determined by flow cytometric analysis using 488nm wavelength laser excitation.
Storage and Stability:
Maybe stored at 4 degrees C before opening. DO NOT FREEZE! Stable at 4 degrees C as an undiluted liquid. Dilute only prior to immediate use. Stable for at least 6 months at 4 degrees C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.