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a-Keto Acid Dehydrogenase Complex, Branched-Chain (BCKDH)

Cat no: K0290

Supplier: United States Biological
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Mitochondial enzymes regulated by reversible phosphorylation. Catalyzes the oxidative decarboxylation of the branched-chain a-keto acids derived from the essential amino acids, leucine, isoleucine and valine. Purified preparations undergo phosphorylation because endogenous kinase is tightly associated with the complex. Specific Activity: ~8-10 mmol of NADH formed per min per mg of protein with a-ketoisovalerate as substrate. Molecular Weight: 3200kD by sedimentation equilibrium centrifugation. Subunit apparent MW: 52kD (E2), 46kD (E1a), 35kD (E1b) Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70 degrees C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: K0290
Size: 10ug
Form: Supplied as a liquid in 50mM Tris HCl, pH 7.0, 14mM b-mercaptoethanol, 1mM benzamidine, 0.1mM PMSF, 1mM EDTA, 10% glycerol.
Purity: ~90% (SDS-PAGE)
References: 1. Methods Enzymol 166: 321

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