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Ubiquitin Conjugating Enzyme (E2N, UBE2N, Ubiquitin-conjugating Enzyme E2 N, Ubiquitin-protein Ligase N, Ubiquitin carrier protein N, Ubc13, Bendless-like ubiquitin-conjugating enzyme, BLU)

Cat no: U1001-01A

Ubiquitin Conjugating Enzyme (E2N, UBE2N, Ubiquitin-conjugating Enzyme E2 N, Ubiquitin-protein Ligase N, Ubiquitin carrier protein N, Ubc13, Bendless-like ubiquitin-conjugating enzyme, BLU)

Ubiquitin-conjugating enzyme E2N (UBC13) is a 16kD enzyme which is an important component of the TLR (Toll-like receptor) and IL-1R signalling pathway. \n\nUBC13 is DNA-damage inducible protein which forms part of an enzyme complex, with UEV1A, that indirectly activates TAK1 via the ubiquitination of TRAF-6. Ubiquitin modification of proteins is important for error-free DNA repair and for targeting of short-lived or abnormal proteins for degradation.\n\nApplications:\nSuitable for use in Immunohistochemistry (paraffin) and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nImmunohistochemistry (paraffin): 2ug/ml\nWestern Blot: 0.5-1ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

U1001-01A

Size

100ug

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid, 0.02% sodium azide.

P Type

Pab

Purity

Purified by Ion Exchange chromatography.

Isotype

IgG

References

1. Chen, Z.J. (2005) Ubiquitin signalling in the NF-kappaB pathway. Nature Cell Biology. 7: 758-766.

Additional Info

Recognizes C-terminal region of human UBC13. Species Crossreactivity: Rat, Mouse.

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SUPPLIER INFO

United States Biological

Applications

ELISA

Reactivities

Hum

More info

Applications

IF

Hosts

Mouse

More info

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, IHC, WB

Hosts

Mouse

More info

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum

More info

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

More info
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