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TGase 2 (Transglutaminase 2 (C polypeptide, protein-glutamine-gamma-glutamyltransferase), G-ALPHA-h, GNAH, Protein-glutamine gamma-glutamyltransferase 2, TG(C), TG2, TGM2, TGase C, TGase H, TGC, Tissue transglutaminase, Transglutaminase-2, Transglutaminas

Cat no: T8260

TGase 2 (Transglutaminase 2 (C polypeptide, protein-glutamine-gamma-glutamyltransferase), G-ALPHA-h, GNAH, Protein-glutamine gamma-glutamyltransferase 2, TG(C), TG2, TGM2, TGase C, TGase H, TGC, Tissue transglutaminase, Transglutaminase-2, Transglutaminas

Transglutaminase, type II, also known as tissue transglutaminase, is a Ca 2+ dependent enzyme that crosslinks proteins by catalyzing the formation of covalent (gamma-glutamyl) lysine isopeptide bonds.1 Induction and activation of type II transglutaminase has been observed during apoptosis and its ability to bind GTP has been correlated with cell cycle progression.2 Site directed mutagenesis of human type II transglutaminase showed that cysteine 277 is essential for its transglutaminase activity but not for its GTPase activity.3 Retenoic acid, an inducer of either differentiation or apoptosis in many different cell lines, is a potent inducer of type II transglutaminase. Transformation of 3T3 fibroblasts with a ras oncogene blocked induction of transglutaminase by retenoic acid, suggesting a role for Ras in regulation of transglutaminase expression and/or activity\n\nApplications: Suitable for use in Western Blotting, Immunoprecipitation.\n\nRecommended Dilution:\nWestern Blot: A 1:1000 dilution detected type II transglutaminase in murine liver, testes and thymus tissue extracts. Tested using murine spleen and kidney extracts as well as the purified enzyme from guinea pig liver. \n\nImmunoprecipitation: 10ul of the lot immunoprecipitated type II transglutaminase from murine liver and spleen tissue extracts but not from kidney extract.

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SPECIFICATIONS

Catalog Number

T8260

Size

250ul

Applications

IP, WB

Hosts

Goat

Reactivities

Mouse, GP

Form

Supplied as a solution in 0.05% sodium azide.

P Type

Pab

Purity

Neat serum

Isotype

IgG

References

1. Lu, S., et al., J. Biol. Chem. 270: 9748, 1995. 2. Piacentini, M., et al., Curr. Top. Microbiol. Immunol. 200: 163, 1995. 3. Lee, K.N., et al., Biochem. Biophys. Acta 1202: 1, 1993. 4. Kosa, K., et al., Biochem. Biophys. Res. Commun. 196: 1025, 1993.

Additional Info

Recognizes type II transglutaminase, Mr ~ 78kDa. of mouse and guinea pig.

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Applications

ELISA

Reactivities

Hum

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Applications

IF

Hosts

Mouse

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Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, IHC, WB

Hosts

Mouse

More info

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum

More info

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

More info
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