HRD1, a Ubiquitin-protein ligase (E3), is required for the degradation and ubiquitination of several endoplasmic reticulum-associated degradation (ERAD) substrates and physically associates with relevant ubiquitin-conjugating enzymes (E2s). Human HRD1 is localized to the ER and ubiquitinates its substrates. HRD1 has been suggested to be up-regulated to protect against ER stress-induced apoptosis by degrading unfolded proteins accumulated in the ER. Mice overexpressing this enzyme develop spontaneous arthropathy. Hrd1 is suggested to be a novel causative factor for arthropathy by triggering synovial cell outgrowth through its antiapoptotic effects.
Applications:
Suitable for use in Western Blot, ELISA and Immunohistochemistry. Other applications not tested.
Recommended Dilution:
ELISA: 1:1000
Western Blot: 1:100-1:500
Immunohistochemistry (Formalin fixed paraffin embedded): 10ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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