Synphilin-1 is a cytoplasmic protein which associates with alpha-synuclein in vivo. Alpha-Synuclein is a major component of lewy bodies in patients with sporadic Parkinson's disease (PD) and mutations of alpha-synuclein are involved in familial forms of PD. Reports suggest that synphilin-1 may play a role in lewy body formation in association with alpha synuclein.
Applications:
Suitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications not tested.
Recommended Dilution:
Immunohistochemistry (paraffin): 1:400
ELISA: 1:5,000-1:10,000
Western Blot: 1:2,000
Optimal dilutions to be determined by the researcher.
Positive Control Tissue:
Human Brain
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.