Syndecan-1 is a transmembrane (Type I) heparan sulfate proteoglycan and is a member of the syndecan proteoglycan family. The syndecans mediate cell binding, cell signaling, and cytoskeletal organization. Bearing properties of both heparan sulfate and chondroitin sulfate, Syndecan-1 functions as a cell surface proteoglycan membrane protein and participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. Altered Syndecan-1 expression has been detected in several different tumor types.
Applications:
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilutions:
Western Blot: 1:1000 using mouse liver lysate. Proteins were visualized using a IgG (HRP) Dk x Rb and chemiluminescence detection. A dimer of Syndecan-1 was identified at ~77kD while monomeric Syndecan-1 is visible at ~32kD. The mature form, with heparan and chondroitin sulfate addtions, can sometimes be seen between 500-1000kD but may need to be solubilized from whole tissues by urea extraction. Triton X-100 or other mild detergents are enough to release the immature precursor forms.
Optimal dilutions to be determined by the researcher.
Recommended Secondary Antibodies:
I1904-46E: IgG, H&L (HRP) Pab Dk x Rb
I1904-46K: IgG, H&L, X-Adsorbed (HRP) Pab Dk x Rb
I1903-89G5: IgG, H&L, X-Adsorbed (HRP) Pab Dk x Rb
I1904-45P6: IgG, H&L (HRP) Pab Dk x Rb
Positive Control:
Mouse liver lysate
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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