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Synaptotagmin, phosphorylated (Ser309) (SYT, Synaptotagmin-1, SYT1, Synaptotagmin I, SytI, p65, SVP65)

Synaptotagmin, phosphorylated (Ser309) (SYT, Synaptotagmin-1, SYT1, Synaptotagmin I, SytI, p65, SVP65)

Cat no: S9108-99P

Supplier: United States Biological
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Neurotransmitter release is mediated by exocytosis of synaptic vesicles at the presynaptic active zone of nerve terminals (1). Synaptic vesicles undergo a trafficking cycle to support rapid and repeated cycles of neurotransmitter release. Calcium-triggered exocytosis is the focal point of the vesicle cycle, which is followed by different routes of endocytosis and recycling. Recycling then leads to the docking and priming of the vesicles for another round of exocytocis and endocytosis. The synaptotagmins are integral membrane proteins of synaptic vesicles. Synaptotagmins may serve as the calcium sensors that trigger neuortransmitter release (2,3). More than 13 synaptotagmins have been described, and synaptotagmin I is among the best characterized isoforms. Synaptotagmin I is an abundant constituent of synaptic vesicles that binds calcium ions through two C2 domains. It is thought that synaptotagmin I triggers exocytosis in a calcium dependent manner, and may also play a key role in endocytosis following membrane fusion. Synaptotagmin is phosphorylated by multiple protein kinases, and these phosphorylation events may play a regulatatory role in both exocytotic and endocytotic events. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Optimal dilutions to be determined by the researcher. Positive Control: Rat brain Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: S9108-99P
Reactivities: Human, Mouse, Rat
Hosts: Rabbit
Applications: Western Blot
Size: 100ul
Form: Supplied as a liquid in 10mM HEPES (pH 7.5), 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
P type: Pab
Isotype: IgG
Purity: Purified by immunoaffinity chromatography.
Alternative names: DKFZp781D2042
References: 1. Sudhof TC. 2004. The synaptic vesicle cycle. Annu Rev Neurosci. 27:509-47. 2. Bai J and Chapman ER. 2004. The C2 domains of synaptotagmin--partners in exocytosis. Trends Biochem Sci. 3:143-51. 3. Chapman, E. 2002. Synaptotagmin: A Ca2+ sensor that triggers exocytosis. Nature Rev Mol Cell Biol. 3:498-508.
Additional info: Recognizes human Synaptotagmin, phosphorylated at (Ser309). Species crossreactivity: mouse and rat.

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