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Synaptophysin 2 (DKFZp686G0883, MGC26651, Synaptoporin, SPO, SYNPR)

Cat no: S9108-25


Supplier: United States Biological
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Synaptophysin 2, also known as Synaptoporin, is a component of the synaptic vesicle membrane and thought to play an important role in synaptic vesicle trafficking (Dai et al., 2003). It is highly homologous to Synaptophysin 1, yet differs in expression. Whereas Synaptophysin 1 is ubiquitiously expressed, Synaptophysin 2 is concentrated in the mossy fiber synapses of the hippocampus (Grabs et al., 1994; Singec et al., 2002). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Positive Control: Rat hippocampal lysate Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: S9108-25
Reactivities: Mouse, Rat
Hosts: Rabbit
Applications: Western Blot
Size: 100ul
Form: 10 mM Hepes (pH7.5), 150 mM NaCl, 100 ug/ml BSA and 50% glycerol
P type: Pab
Purity: Purified by immunoaffinity chromatography.
References: Dai J, Ji C, Gu S, Wu Q, Wang L, Xu J, Zeng L, Ye X, Yin G, Xie Y, Mao Y (2003) Cloning and sequence analysis of the human cDNA encoding the synaptoporin, a highly conserved synaptic vesicle protein. Molecular Biology Reports 30:185-191.Singec I, Knoth R, Ditter M, Hagemeyer CE, Rosenbrock H, Frotscher M, Volk B (2002) Synaptic vesicle protein synaptoporin is differently expressed by subpopulations of mouse hippocampal neurons. J Comp Neurol 452(2):139-153.Grabs D, Bergmann M, Schuster T, Fox PA, Brich M, Gratz M (1994) Differential expression of synaptophysin and synaptoporin during pre- and postnatal development of the rat hippocampal network. Eur J Neurosci. 6(11): 1765-71
Additional info: The antibody has been demonstrated to work in rat. It is anticipated that the antibody will work in mouse based on the fact that this species has 100% homology with the amino acid sequence used as antigen

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