Synapsin I is a member of neuron-specific phosphoproteins that associate with synaptic vesicles (1). Three mammalians synapsins have been identified, Synapsin I, II and III. Generated by differential splicing, Synapsin Ia and Synapsin Ib differ in their Carboxyl termini (2). Synapsin I also functions in binding synaptic vesicles to several elements of the cytoskeleton including actin filaments, microtubules and spectrin. (3) Fairly abundant protein of the brain (0.4%), Synapsin I is best known to be CaM kinase II substrate in addition to cAMP-dependent protein kinase. (4) Phosphorylation at site Ser 566 and Ser 603 by CaM kinase II will cause a 5 fold decrease in the affinity of synaptic vesicles binding. A phosphorylation of serine 9 on synapsin amino-terminal can lead to inhibition in its binding to phospholipids and dissociation of synapsins from synaptic vesicles.
Applications:
Suitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested.
Recommended Dilution:
Western Blot: 1:1000-5000
Immunohistochemistry: 1:250
Immunocytochemistry: 1:50
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Manufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
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