Syk is a 80kD protein tyrosine kinase (PTK), expressed in immature T cells, all B cells, basophils, hepatocytes, platelets, and erythrocytes. It functions in some T cell receptor signaling processes in addition to B cell receptor and integrin-mediated signal transduction processes. Syk-/syk- mice exhibit defective phagocytosis by macrophages.
Applications:
Suitable for use in Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution:
Immunohistochemistry: 1:200 for 10min at RT
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10min followed by cooling at RT for 20min
Optimal dilutions to be determined by the researcher.
Positive Control:
Raji cells, lymphoma
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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