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Syk, phosphorylated (Tyr525/526) (Spleen tyrosine kinase, Tyrosine-protein kinase SYK)

Syk, phosphorylated (Tyr525/526) (Spleen tyrosine kinase, Tyrosine-protein kinase SYK)

Cat no: S9100-01G

Supplier: United States Biological
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Syk, a non-receptor tyrosine kinase, belongs to the Syk-Zap70 family of protein tyrosine kinase (PTK) and is involved in a wide variety of cellular functions including the pathogenesis of malignant cancer (1). Ubiquitously expressed in hematopoietic cells, Syk has been found to be an effector of B cell receptor (BCR) and functions in B & T cell lymphopoiesis. In the absence of Syk, B cell development is blocked (2). Upon binding to the BCR and to the cell surface receptor of hematopoietic cells (3), Syk is activated and phosphorylates Phospholipase C resulting in the downstream activation of ERK & JNK kinase. Syk also phosphorylates PI3-K activating the Akt pathway (4). Cross-linking of the T cell antigen receptor (TCR)-CD3 complex induces rapid tyrosine phosphorylation and then activation of Syk, which in turn phosphorylates a multitude of intracellular substrates such as Cbl (5). Substitution of Tyr-525 and Tyr-526 at the autophosphorylation site of Syk in mCD8-Syk substantially reduced the kinase activity and the binding to PLC-gamma1 SH2(C) in vitro (6). Applications: Suitable for use in Western Blot, Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:5000-1:10,000 Immunocytochemistry: 1:100-1:250 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Manufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
Catalogue number: S9100-01G
Reactivities: Human
Hosts: Rabbit
Applications: Immunohistochemistry, Western Blot
Size: 100ul
Form: Supplied as a liquid in 50 mM Tris-Glycine, pH 7.4, 0.15 M sodium chloride, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
P type: Mab
Isotype: IgG
Purity: Supernatant
Alternative names: Spleen tyrosine kinase
References: 1. Bolen, J. B., et al. 1997. Annu. Rev. Immunol. 15:371 2. Turner M, et al. Immunol Today 2000, 21:148-154. 3. Reth, M.. 1989. Nature 338:383. 4. Jiang A, et al: J Exp Med 1998, 188:1297-1306. 5. Latour, S et al. (1996) J. Biol. Chem. 271, 22782-90 6. Law CL, et al. Mol Cell Biol 16(4), 1305-15, 1996
Additional info: Recognizes human Syk phosphorylated at Tyrosine 525/526.

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