SUZ12 (suppressor of zeste 12; also Joined to JazF1) is a 90-95kD member of the Polycomb group of transcriptional regulators. It is part of a transcription repression complex termed PRC2 (Polycomb repressive complex 2) that methylates histones in the nucleus, resulting in homeotic (pattern-inducing) gene silencing. Human SUZ12 is 739 amino acids (aa) in length. It contains one C2H2-type zinc finger region (aa 44-8471) and a C-terminal VEFS-box (aa 563-639). There is one potential splice form that shows a deletion of 51 aa over aa 129-197. Human SUZ12 shares 90% and 99% aa identity with mouse and dog SUZ12, respectively.
Applications:
Suitable for use in Direct ELISA, Western Blot, and Immunocytochemistry. Other applications not tested.
Recommended Dilution:
Direct ELISA: 0.5-1.0ug/ml detection limit for rhSUZ12 is ~ 5ng/well.
Western Blot: 1-2ug/ml detection limit for rhSUZ12 is ~ 25ng/lane under non-reducing and reducing conditions.
Immunocytochemistry: 10ug/ml using WS-1 human dermal fibroblasts. Cells were fixed with PBS containing 4% paraformaldehyde for 20 minutes at room temperature and blocked with PBS containing 10% normal donkey serum, 0.1% Triton(R) X-100, and 1% BSA for 45 minutes at room temperature. After blocking, cells were incubated with diluted primary antibody overnight at 4 degrees C followed by fluorochrome-coupled anti-mouse IgG at room temperature in the dark for one hour. Between each step, cells were washed with PBS containing 0.1% BSA.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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