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SUMO-1 Activating Enzyme, Subunit 1 (SAE1, AOS1, FLJ3091, HSPC140, Sentrin/SUMO-activating Protein AOS1, SUA1, SUMO1 Activating Enzyme E1 N Subunit, Ubiquitin-like 1-activating Enzyme E1A, UBLE1A, Ubiquitin-like Protein SUMO1 Activating Enzyme)

Cat no: S8025-85G

SUMO-1 Activating Enzyme, Subunit 1 (SAE1, AOS1, FLJ3091, HSPC140, Sentrin/SUMO-activating Protein AOS1, SUA1, SUMO1 Activating Enzyme E1 N Subunit, Ubiquitin-like 1-activating Enzyme E1A, UBLE1A, Ubiquitin-like Protein SUMO1 Activating Enzyme)

SAE1 is a 35.5kD nuclear protein belonging to Ubiquitin E1 family. SAE1 acts as an E1 activating enzyme for SUMO1, SUMO2 and SUMO3. It mediates ATP-dependent activation of SUMO proteins and formation of a thio-ester linkage with a conserved cysteine residue on SAE2. SAE1 is broadly expressed in a wide range of tissues.\n\nApplications:\nSuitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nImmunohistochemistry (paraffin): 5ug/ml\nELISA: 1:5,000-1:20,000\nWestern Blot: 1:500-1:2,000\nOptimal dilutions to be determined by the researcher.\n\nPositive Control Tissue: \nHuman spleen\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

S8025-85G

Size

50ug

Applications

ELISA, IHC, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

Additional Info

Recognizes human SUMO-1 Activating Enzyme Subunit 1.

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SUPPLIER INFO

United States Biological

Applications

ELISA

Reactivities

Hum

More info

Applications

IF

Hosts

Mouse

More info

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, IHC, WB

Hosts

Mouse

More info

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum

More info

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

More info

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