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Sulfatase Modifying Factor 2 (SUMF2)

Cat no: S8016-11

Supplier: United States Biological
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Sulfatase Modifying Factor 2 (SUMF2) is structurally similar to SUMF1, which activates sulfatases by converting their active site residue cysteine to formylglycine. Functionally, SUMF2 does not activate sulfatases, but rather competes with SUMF1 for the same substrates. The amino acid sequence of mouse SUMF2 is 94%, 83%, 82%, 81% and 72% identical to that of rat, human, canine, chimpanzee and chicken. Applications: Suitable for use in Direct ELISA and Western Blot. Other applications not tested. Recommended Dilution: Direct ELISA: 0.5-1ug/mL detection limit for rmSUMF2 ~ 5ng/well. Western Blot:1-2ug/mL detection limit for rmSUMF2 ~ 5ng/lane under non-reducing and reducing conditions. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20 degrees C. Reconstitute to nominal volume by adding sterile 40-50% glycerol, PBS. Aliquot and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: S8016-11
Reactivities: Mouse
Hosts: Rat
Applications: ELISA, Western Blot
Size: 100ug
Form: Supplied as a lyophilized powder in PBS with 5% trehalose. Reconstitute with 200ul 40-50% glycerol, PBS.
P type: Mab
Isotype: IgG2a
Purity: Purified by Protein G affinity chromatography.
Additional info: Recognizes mouse SUMF2.

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