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Stromal Interaction Molecule 1 (STIM1, D11S4896E, GOK)

Cat no: S7975-88A3

Supplier: United States Biological
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STIM1 (Stromal Interaction Molecule 1; GOK) is an essential and conserved component of store-operated Ca2+ channel function. It is a Ca2+ sensor that activates CRAC channels and migrates from the Ca2+ store to the plasma membrane (STIM1 translocates from the endoplasmic reticulum to the cell membrane in response to a depletion of intracellular calcium). STIM1 is a typical transmembrane protein with an extracellular N-terminal domain and a cytosolic C-terminal domain. The protein is highly hydrophobic with only a short region of hydrophobicity that likely represents the transmembrane region. The mouse STIM1 homologue (Stim1) was identified independently as a stromal-cell-derived gene product that binds to the surface of haematopoietic cells, particularly pre-B lymphoid cells, and can promote subsequent clonal expansion. Murine Stim1 is 96% identical with human STIM1 in amino acid sequence, and maps to a region of mouse chromosome 7 syntenic with human 11p15.5. Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Western Blot: Reducing and non-reducing conditions. Optimal dilutions to be determined by the researcher. Positive Control: RBL rat basophilic leukemia cell line Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: S7975-88A3
Reactivities: Mouse, Rat
Hosts: Mouse
Applications: Immunoprecipitation, Western Blot
Size: 100ug
Form: Supplied as a liquid in PBS, pH 7.4.
P type: Mab
Isotype: IgG1
Purity: Purified by Protein A affinity chromatography.
References: 1. Williams RT, Manji SS, Parker NJ, Hancock MS, Van Stekelenburg L, Eid JP, Senior PV, Kazenwadel JS, Shandala T, Saint R, Smith PJ, Dziadek MA.: Identification and characterization of the STIM (stromal interaction molecule) gene family: coding for a novel class of transmembrane proteins. Biochem J. 2001 Aug 1;357(Pt 3):673-85. 2. Draber P, Draberova L.: Lifting the fog in store-operated Ca2+ entry. Trends Immunol. 2005 Dec;26(12):621-4.
Additional info: Recognizes mouse and rat STIM1, a 84kD essential and conserved component of store-operated Ca2+ channel function.

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