Streptavidin is a tetrameric protein from Streptomyces avidinii that binds very tightly to the vitamin biotin with a Kd of ? 10-14 mol/L. The high-affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits.
Applications:
Suitable for use in ELISA, Western Blot and Dot Blot. Other applications not tested.
Recommended Dilution:
ELISA: 1:100,000-1:500,000
Western Blot: 1:100,000-1:500,000
Dot Blot: 1:100,000-1:500,000
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.