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Cat no: S7973-71B

Supplier: United States Biological
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Streptavidin is a tetrameric protein from Streptomyces avidinii that binds very tightly to the vitamin biotin with a Kd of ? 10-14 mol/L. The high-affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits. Applications: Suitable for use in ELISA, Western Blot and Dot Blot. Other applications not tested. Recommended Dilution: ELISA: 1:100,000-1:500,000 Western Blot: 1:100,000-1:500,000 Dot Blot: 1:100,000-1:500,000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: S7973-71B
Hosts: Rabbit
Applications: ELISA, Western Blot
Size: 10mg
Form: Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide.
P type: Pab
Isotype: IgG
Purity: Purified by Protein G affinity chromatography.
Additional info: Specific to streptavidin.

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