Home  >  Products  >  STK35 (Serine/Threonine-protein Kinase 35, CLP-36-interacting Kinase 1, CLIK-1, PDLIM1-interacting Kinase 1, Serine/Threonine-protein Kinase 35 L1, CLIK1, PDIK1, STK35L1)
STK35 (Serine/Threonine-protein Kinase 35, CLP-36-interacting Kinase 1, CLIK-1, PDLIM1-interacting Kinase 1, Serine/Threonine-protein Kinase 35 L1, CLIK1, PDIK1, STK35L1)

STK35 (Serine/Threonine-protein Kinase 35, CLP-36-interacting Kinase 1, CLIK-1, PDLIM1-interacting Kinase 1, Serine/Threonine-protein Kinase 35 L1, CLIK1, PDIK1, STK35L1)

Cat no: S7973-58Z3

Supplier: United States Biological
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STK35 associates with the PDZ-LIM protein CLP36 through the LIM domain and undergoes autophosphorylation in vitro. STK35 localizes predominantly to the nucleus; however, cotransfection of STK35 with CLP36 demonstrated redistribution of STK35 to the cytoplasm and into actin stress fibers, where it colocalizes with CLP36. The presence of STK35 disrupted the periodic staining pattern of CLP36 in these fibers. It is suggested that CLP36 specifically targets STK35 to actin stress fibers and that STK35 may represent a regulator of the actomyosin cytoskeleton in nonmuscle cells. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1-3ug/ml Immunohistochemistry (paraffin): 10ug/ml Optimal dilutions to be determined by the researcher. Positive Control: Human Testis lysate Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: S7973-58Z3
Reactivities: Human
Hosts: Goat
Applications: Immunohistochemistry, Western Blot
Size: 100ug
Form: Supplied as a liquid in Tris saline, 0.02% sodium azide, pH7.3, 0.5% BSA.
P type: Pab
Isotype: IgG
Purity: Purified by immunoaffinity chromatography.
References: 1. Vallenius T, Makela TP. Clik1: a novel kinase targeted to actin stress fibers by the CLP-36 PDZ-LIM protein. J Cell Sci. 2002 May 15;115(Pt 10):2067-73. PMID: 11973348. 2. Goyal P. et al., Identifying and characterizing a novel protein kinase STK35L1 and deciphering its orthologs and close-homologs in vertebrates. PLoS One. 2009 Sep 16;4(9):e6981. PMID: 19756140.
Additional info: Recognizes human STK35. Species sequence homology: mouse and rat.

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