Six-transmembrane epithelial antigen of the prostate (STEAP) is expressed predominantly in human prostate tissue and in other common malignancies including prostate, bladder, colon, and ovarian carcinomas, and in Ewing's sarcoma, suggesting that it could function as an almost universal tumor antigen. Immunohistochemical analysis indicated STEAP-1 localization at cell-cell junctions of the secretory epithelium of prostate and prostate cancer cells. The favorable expression profiling of STEAP-1 in normal and cancer tissues suggested its potential use as a target for immunotherapy.
Applications:
Suitable for use in Immunofluorescence, ELISA and Western Blot. Other applications not tested.
Recommended Dilution:
Immunofluorescence: 1:100-1:300
Western Blot: 1:500-1000
Optimal dilutions to be determined by the researcher.
Hybridoma:
Hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human STEAP1 protein.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.