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Stathmin, phosphorylated (Ser62)

Cat no: S7972-50B

Supplier: United States Biological
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Stathmin (oncoprotein 18, op18) is a ubiquitous cytosolic phosphoprotein with various regulatory functions in cell proliferation, differentiation signaling, and activation (1). In particular, stathmin is involved in the regulation of tubulin dynamics through inhibition of microtubule formation and/or microtubule depolymerization. Stathmin interacts with soluble tubulins (alpha, beta-tubulin) resulting in the formation of T2S complex which sequesters free tubulin, impeding tubulin formation (2). Stathmin activity is regulated through phosphorylation at Ser15, Ser24, Ser37 and Ser62 by various protein kinases (e.g. MAP-kinase, p34cdc2 kinase), weakening stathmins affinity to tubulin (3). A mutation on stathmin can lead to excess build up of mitotic spindle and with possible consequence of unregulated cell cycles seen in cancer cells (4). Stathmin is also known as the generic member of a protein family which includes neural proteins SCG10, SCLIP and RB3/RB3'/RB3". All members in this family exhibits tubulin binding ability. Applications: Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:2,500-10,000 Immunohistochemistry: 1:250-1:500 Immunoprecipitation: 1:20 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Manufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
Catalogue number: S7972-50B
Reactivities: Human
Hosts: Rabbit
Applications: Immunohistochemistry, Immunoprecipitation, Western Blot
Size: 100ul
Form: Supplied as a liquid in 50 mM Tris-Glycine, pH 7.4, 0.15 M sodium chloride, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
P type: Mab
Isotype: IgG
Purity: Supernatant
Alternative names: Phosphoprotein p19, pp19, Leukemia-associated phosphoprotein p18, Oncoprotein 18, Op18, pp17, Prosolin, Metablastin, Protein Pr22
References: 1. Cassimeris L et al. Curr Opin in Cell Bio. 14:18-24, 2002. 2. Cle?ment MJ et al. Biochemistry. 44:14616-14625, 2005. 3. Jourdain L et al. Biochemistry 36:10817-10821, 1997. 4. Moreno FJ, Avila J, Mol Cell Biochem 183: 201-209, 1998.
Additional info: Recognizes human Stathmin phosphorylated on Serine 62.

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