Signal Transducer and Activator of Transcription 6 (STAT6) is 119kD mediator of transcription in response to IL-4 and IL-13 stimulation. It resides in the cytoplasm in a latent form as a monomer protein. Active IL-4 engages SH2 binding domain of STAT6 that results in STAT6 phosphorylation at Tyr641 residue. Phosphorylated monomeric STAT6 dimerize via their N- terminal domain. Consequently dimeric STAT6 is translocated into the nucleus, binds target DNA sequence, and promotes transcription. It was demonstrated that normal STAT6 function is critical for commitment of CD4+ T cells to the Th2 phenotype and IgE response. Thus, mice deficient in STAT6 failed to produce IgE, were deficient in Th2 differentiation, and did not show allergic airway response.
Applications:
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution:
Western Blot: 0.5-2ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.